Serum-free and chemically defined mesenchymal stromal cell culture
Using serum-containing media limits the impact of your research on human mesenchymal stromal cells (hMSC) in non-clinical and clinical applications, and impedes the reproducibility of your results. You are interested in reducing or completely eliminating serum content in your media, however cell performance has suffered. You have tried a few coatings or media supplements, but so far there is no clear solution to this issue. Ideally, you want the reliability of more chemically defined media, while maintaining the performance of your hMSC cultures.
denovoMATRIX provides a chemically defined cell adhesion-promoting microenvironment offered as pre-coated cultureware, specially formulated for expansion of human mesenchymal stromal cells (hMSC) in serum-free and xeno-free media.
Chemically defined coating
Serum-free and xeno-free hMSC culture
Storage at room temperature
Chemically defined culture with myMATRIX MSC
Animal and human component-free
Facilitates translation of your research into clinical applications
Our coating is manufactured without any animal or human components. Due to the lot-to-lot consistency our product may be used without batch validation. Hence, using myMATRIX MSC saves you time and enhances the reproducibility of your cell culture experiments. Moreover, our GMP-certifiable coating enables the transfer of established processes from research to cell therapy development.
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Ready-to-use: coating preparation time eliminated
Reduce contamination risk by 90 %
Free up fridge space: myMATRIX MSC is stored at room temperature
Compatible with various reagents and media
denovoMATRIX aims to streamline your cell culture process. Our products are manufactured and shipped as ready-to-use cultureware. No elaborate coating, washing steps or dedicated solutions necessary, which also reduces the risk of contamination. You can store them at room temperature for up to one year before usage, and don’t have to worry about space in your fridge or cold storage room. Use myMATRIX MSC in combination with many dissociation reagents, media and supplements.
Microenvironment recreated de novo
Optimized composition for serum-free culture of hMSC
Adhesion peptide sequence enables integrin-specific cell interaction
Glycosaminoglycan-mimetic facilitates paracrine signaling
denovoMATRIX technology incorporates biologically relevant polymers to recreate essential elements of the cell microenvironment. One of them includes the synthetic peptide sequence ‘RGD’ that facilitates cell adhesion by stimulating integrin receptors of the cells. In addition, myMATRIX MSC contains a sulfated polysaccharide, which mimics the function of glycosaminoglycans (GAG). In vivo, GAGs are instrumental in stabilizing and storing soluble factors. As an example, the Fibroblast Growth Factor (FGF-2) is stabilized over 24 hours on myMATRIX MSC. Thus, the myMATRIX MSC replicates the in vivo environment more accurately than existing technologies. myMATRIX MSC is tailored to create optimal expansion conditions for human mesenchymal stromal cells (hMSC), also termed mesenchymal stem cells.
Accellerate proliferation with myMATRIX MSC
- Increased cell doubling by 50 %
The stable and efficient expansion of human mesenchymal stromal cells (hMSC), also termed human mesenchymal stem cells, in a short time period remains challenging. The risk of spontaneous transformation, inflammatory reactions and deterioration of potency with long-term expansion affects basic as well as clinical research. myMATRIX MSC promotes cell adhesion and supports enhanced cell proliferation in serum-containing as well as serum-free media. It considerably reduces doubling times and thereby increases the number of cell population doublings within a given passage.
Figure 1. The culture of hMSCs on myMATRIX MSC results in enhanced cell proliferation.
Consistently high cell viability with myMATRIX MSC
- Cell viability above 90 % with low viability variance
In vitro cell viability is influenced by many factors such as media, supplements, dissociation reagents and surface adhesion. Suboptimal growth conditions and cytotoxic effects eventually lead to cell death, which negatively influences the proliferation and viability of the remaining population. Thus, careful monitoring and maintenance of the cell viability in cell culture is essential. Determining the number of viable cells expanded on myMATRIX MSC during a long-term study revealed consistently high viabilities in serum-containing as well as serum-free conditions.
Figure 2. hMSC cultured on myMATRIX MSC show consistently high viabilities.
Maintain characteristic marker expression with myMATRIX MSC
High expression of CD73, CD90 and CD105 antigens in 99 % of the hMSC
One minimal criterion for in vitro cultures of human mesenchymal stromal cells (hMSC) is the expression of surface markers CD73, CD105 and CD90 in at least 95 % of the cell population as defined by the International Society for Cellular Therapy. hMSC, also termed mesenchymal stem cells cultured on myMATRIX MSC in serum-free as well as serum-containing medium maintained their characteristic surface marker expression for ten consecutive passages.
Figure 3. Over 99 % of hMSCs cultured on myMATRIX MSC are positive for the markers CD73, CD90 and CD105.
Maintain characteristic morphology with myMATRIX MSC
Morphology is an integral aspect of the phenotype of a cell and is defined by its cytoskeleton. Changes in cell morphology are not only connected to external stimuli but also coordinated with cell growth or apoptosis. Human mesenchymal stromal cells (hMSC), also termed human mesenchymal stem cells, cultured on myMATRIX MSC maintain their characteristic fibroblast-like morphology. As previously described in the literature and by media suppliers for cells expanded in serum-free conditions, hMSC cultured on myMATRIX MSC also acquire a more compact spindle-shaped morphology. In contrast, cells grown in media with serum develop a flattened and spread shape.
Figure 4. hMSC cultured on myMATRIX MSC display the characteristic fibroblast-like morphology.
Maintain multilineage potential with myMATRIX MSC
Tri-lineage differentiation potential into adipocytes, chondrocytes and osteocytes
Human mesenchymal stromal cells (hMSC) are considered a promising stem cell source for tissue engineering and regenerative medicine due to their capacity to differentiate into various lineages. The functional multipotency of hMSC cultured on myMATRIX MSC in the presence or absence of serum was confirmed by their in vitro differentiation into the three specific mesoderm lineages: adipocytes, chondrocytes and osteocytes.
Figure 5. hMSC cultured on myMATRIX MSC show differentiation capacity into adipocytes, chondrocytes and osteocytes.