In vitro, culture conditions are usually extremely simplified with respect to representing the natural function of the ECM. Generally, in vitro cell culture serves a minimalist approach that utilizes single ECM proteins or RGD-containing peptides to stimulate integrin binding and therefore cell adhesion.
denovoMATRIX biomaterials combine specific peptides and polysaccharide chains of the glycosaminoglycan type to recapitulate the key functions of the cell niche in vitro. While the peptides facilitate integrin binding and signaling, the glycosaminoglycans act as growth factor reservoirs and thus support cell adhesion and signaling.
Each cell, whether it is a stem cell or a somatic cell, has different requirements and therefore their own specific niche.