We believe that every cell requires its niche.
Cells are dependent on their niche
The extracellular matrix (ECM) is a complex network of proteins and polysaccharide chains that is secreted by cells. Here you see a simplified view of the in vivo ECM that is a key contributor to the cellular microenvironment, also called the cell niche. The ECM is not just an inert structure. It is a dynamic network in which its components are in direct exchange with resident cells. Thus, the ECM serves specific roles, which contribute to cellular function as well as mechanical properties of tissues.
denovoMATRIX recapitulates key functions of the niche
In vitro, culture conditions are usually extremely simplified with respect to representing the natural function of the ECM. Generally, in vitro cell culture serves a minimalist approach that utilizes single ECM proteins or RGD-containing peptides to stimulate integrin binding and therefore cell adhesion.
denovoMATRIX biomaterials combine specific peptides and polysaccharide chains of the glycosaminoglycan type to recapitulate the key functions of the cell niche in vitro. While the peptides facilitate integrin binding and signaling, the glycosaminoglycans act as growth factor reservoirs and thus support cell adhesion and signaling.
Each cell, whether it is a stem cell or a somatic cell, has different requirements and therefore their own specific niche.
denovoMATRIX technology triples lifetime of FGF2 growth factor
Growth factors are part of the cellular microenvironment and are stabilized by the cell surrounding extracellular matrix (ECM) components such as glycosaminoglycans (GAGs).
As denovoMATRIX technology incorporates GAGs, we can provide benefits for stabilizing growth factors in cell culture media by our functional coatings. We could show an extended half-life of basic fibroblast growth factor (FGF2) in comparison to other cell culture coatings. This stabilization supported the maintenance of stemness and promoted the survival of stem cells, especially when applying restricted feeding regimes.
We have incorporated this feature into our myMATRIX products with the aim to improve culture performance, reduces costs and simplify stem cell culture.
LET US DO THE HEAVY LIFTING.
We develop, characterize and manufacture extracellular matrix-mimetic coatings for your applications. denovoMATRIX also offers its expertise in cell line establishment, cell expansion and cell analysis to enhance your research and development projects. Contact us for more information on our service offer.
denovoMATRIX enables biology in vitro
denovoMATRIX has developed a platform technology, which enables the functional recreation of an ECM for any cell type or application. Based on our platform, users can quickly identify key parameters for optimal adhesion, signaling and cell growth. Optimal combinations of our components results in a high performance culture substrate providing multiple benefits dependent on user need.
Our Technology – Your benefits
Maintenance of stemness or their respective specialized function
Prolonged and increased survival
Maintenance of cellular identity and genetic fidelity