Optimal inoculation conditions must be identified separately for each cell type, vessel type/bioreactor system, and microcarrier concentration. Three different regimes have been successfully used depending on the bioreactor system of your choice: static attachment, intermittent agitation, and continuous agitation during the first 12-24 h. To maximize seeding efficiency and allow even cell distribution we advise reducing the initial working volume to achieve a high cell-to-beadMATRIX ratio during the inoculation phase. After the inoculation phase, the working volume can then be increased.