FAQs

Q: What is the recommended harvesting method for MSCs grown on beadMATRIX?

Mesenchymal stromal cells (MSCs) are routinely harvested using detachment reagents such as trypsin-EDTA, TypLE Express Enzym, TrypZean, or collagenase. Detachment reagent concentration and detachment time must be carefully optimized for each culture system to enable maximum recovery of cells while preserving high cell quality. A combination of different detachment reagents and the integration of hydrodynamic forces can facilitate the detachment of the cells from each other as well as from the microcarrier. Note, if cells are growing confluent on the microcarrier it might take longer to detach them, which ultimately could affect their quality.

Q: How to find the optimal beadMATRIX culture procedure?

To achieve a high-quality cell culture, conduct an initial experiment to determine the time for attachment of cells to the microcarriers, the tendency of cells to clump, and the feeding regime. Optimization of these conditions will subsequently result in your ideal cell culture procedure.

Q: What culture conditions should I use for the proliferation phase?

You need to determine the optimal microcarrier concentration and stirring speed depending on your cell type and vessel type/bioreactor system. If you use agitation, we recommend starting with a minimum agitation speed that equally distributes microcarrier and cells in the medium and increasing the speed whenever necessary.

Q: Which inoculation condition results in the highest cell attachment?

Optimal inoculation conditions must be identified separately for each cell type, vessel type/bioreactor system, and microcarrier concentration. Three different regimes have been successfully used depending on the bioreactor system of your choice: static attachment, intermittent agitation, and continuous agitation during the first 12-24 h. To maximize seeding efficiency and allow even cell distribution we advise reducing the initial working volume to achieve a high cell-to-beadMATRIX ratio during the inoculation phase. After the inoculation phase, the working volume can then be increased.

Q: What seeding density shall I use for the inoculation?

The optimal seeding density can vary greatly depending on the individual cells/tissue source. We recommend starting with the seeding density you are using in 2D monolayer culture and optimizing therefrom.

Q: What are the steps that I should perform prior to cell seeding?

Before use, wash the beadMATRIX once with PBS and once with the pre-warmed medium. Discard the supernatant, add the required amount of medium and cells, and start the inoculation phase.

Q: What concentration of beadMATRIX should I use?

The optimal concentration must be adjusted for each cell culture vessel or culture system. As a starting point for microcarrier-based MSC cultures with beadMATRIX, we generally recommend a microcarrier concentration between 1-20 g/L.

Q: Can beadMATRIX be steam sterilized?

beadMATRIX are sterile, polystyrene-based microcarriers aseptically coated with a biomatrix. beadMATRIX microcarriers do not need further sterilization procedures. In order to maintain its high performance and beneficial effects on cell attachment and proliferation it is generally not recommended to steam sterilize beadMATRIX.

Q: How should beadMATRIX be stored?

The beadMATRIX is stable for one year when stored in dry conditions at room temperature.

Q: Can I culture cells in any unused wells of a myMATRIX iPSC plate after performing an experiment?

It is generally recommended to use unopened myMATRIX iPSC cultureware for new experiments. However, if not all wells of the cell culture plate are used, fill unused wells with a basal medium (e.g., DMEM/F12, Advanced DMEM) and leave the medium until use.

We use cookies on different pages in order to make our website more attractive to visitors. By using this website, you consent to the use of cookies in accordance with our privacy policy. Got it